A Novel Photoproduct of 1, 3-Dimethyluracil in Phosphate Buffered-saline

1, 3-Dimethyluracil （DMU） in phosphate buffered-saline （PBS, pH=8） was irradiated by a medium pressure mercury lamp （MPML） and produced a novel compound C6H9N2O6P. The composition and structure of the compound has been identified by elemental analysis, EIMS, UV, IR, ^1H and ^31P-NMR.     

2-取代亚肼基-1,3-二硫杂环戊烷类化合物的合成及其生物活性研究

以取代苯甲醛、取代吡啶和取代苯甲酸酯等为原料合成了 10种含硫杂环化合物 (其中 2a～ 2g化合物为国内外未见报道 ) .在合成过程中采用了超声波技术 ,极大地缩短了反应时间 ,提高了收率 .所得化合物的结构经IR ,1 HNMR ,MS和元素分析证实 .对其生物活性进行了初步测试 ,结果证明一些化合物具有良好的抑菌或杀菌作用 ,其中 2b ,2c ,2e ,2h ,2i和2j对大肠杆菌 ( 10 40 7号 )的抗菌效果与CET和SMZ相当 ;2b ,2c ,2f对草绿色链球菌 ( 112 3号 )的抗菌效果与CET和SMZ相当 .     

Preparation and properties of polymer and quantum dot composites

Quantum dots (QDs) were prepared in an organic system through a simple and low-cost wet chemistry method. Polymer beads with a diameter of 60–70 nm and specific functional groups were synthesized by a particular seeded emulsion polymerization technique. QDs were embedded in the polymer beads with the specific functional groups through dissolving and swelling method, which provided the condition for the conjunction of biomolecules and QDs as fluorescent probes. The prepared composites were characterized with UV-Vis, PL, TEM, FTIR, CLSM and conductance titration etc. The results show that QDs are successfully embedded in polymer beads, which breaks the limitation that the conjunction of biomolecules and QDs can be achieved only for those synthesized in aqueous system. __________ Translated from Journal of Shanghai Jiaotong University, 2005, 39(1) (in Chinese)     

Structural Characterization of Anhydroicaritin Glycosides Using ESI—FT—ICR Mass Spectrometry

Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) was used to determine the structures of anhydroicaritin glycosides by the MS/MS experiments of anhydroicaritin glycosides and their methylated derivatives,With high accuracy FT-ICR-MS provides much information about the structures of compounds ,FT-ICR-MS shows the great potential application in the structural characterization of unknown compounds.     

Solid-supported synthesis of a peptide beta-turn mimetic

[structure: see text]The solid-supported synthesis of a bicyclic diketopiperazine, a potential peptide beta-turn mimetic, is described. The Ugi reaction between the resin ester of alpha-N-Boc-diaminopropionic acid (an amine input), alpha-bromo acid, aldehyde, and isocyanide is the key step in the proposed protocol.     

血清中葡萄糖含量CCQM-K11.1国际比对

研究利用同位素稀释质谱法测定血清中的葡萄糖含量，并利用该方法参加国际物质量咨询委员会（CCQM）2005年组织的关键比对CCQM—K11．1，我国国家标准物质研究中心（NRCCRM）的测定结果与韩国（KRISS）和墨西哥（CENAM）的测定结果非常接近，日本（NMIJ）的测定结果离群偏高。     

Capillary electrophoresis as a tool for screening aptamer with high affinity and high specificity to ricin

Aptamers which specifically recognize targets are selected from random oligonucleotide library using systematic evolution of ligands by exponential enrichment (SELEX). In this paper, capillary electrophoresis (CE) as a separation approach has been introduced to SELEX procedure. The high efficiency of CE gives rise to greatly shorten the selection procedure. The results from enzyme-linked assay and dot blot experiment show that an enrichment pool has been obtained after four rounds selection, which can specifically recognize ricin. __________ Translated from Chemical Journal of Chinese Universities, 2006, 27(10): 1,840–1,843 [译自: 高等学校化学学报]     

Intein-mediated biotinylation of proteins and its application in a protein microarray

We report here the first example using an intein-mediated expression system to generate biotinylated proteins suitable for immobilization onto avidin-functionalized glass slides. With this novel array, proteins are site-specifically immobilized on the glass surface and are able to retain their native activity. The advantage of the avidin/biotin linkage over his-tag/Ni-NTA strategies for protein immobilization is highlighted by its ability to withstand a variety of chemical conditions, which makes this new protein array compatible with most biological assays.     

含硫希夫碱的合成研究

合成了一种含硫希夫碱—1，4-双[(2’-甲醛缩硫代氨基脲基)苯氧甲基]乙烷及其中间产物1，4-双[(2’-甲醛)苯氧甲基]乙烷．其结构经元素分析，IR和1H NMR表征。     

Reversed-phase liquid chromatography of proteins and peptides using multimodal copolymer-encapsulated silica

Multimodal copolymer-encapsulated particles for liquid chromatography were prepared by bonding 1-octadecene and unsaturated carboxylic acids on silica particles (5 microm diameter, 300 A pores) for liquid chromatography of proteins. These multimodal copolymer-encapsulated particles can provide both hydrophobic and hydrogen bonding interactions with polar compounds. The chromatographic performance of these multimodal copolymer-encapsulated particles for peptide and protein separations was evaluated under reversed-phase conditions. Compared with typical C8-bonded silica, polymer-encapsulated particles were more stable in acidic mobile phases and provided better recoveries, especially for large proteins (Mr>0.5 x 10(6)). Totally hydrophobic polymer-encapsulated particles were found to produce broad peaks for proteins, and significant improvements were observed by introducing hydrophilic groups (-COOH) onto the polymer-encapsulated surface to form a multimodal phase. For the reversed-phase liquid chromatography of peptides and proteins, improved selectivity and increased solute retention were found using the multimodal polymer-encapsulated particles. More peaks were resolved for the separation of complex peptide mixtures such as protein digests using the multimodal polymer-encapsulated particles as compared to totally hydrophobic polymer-encapsulated particles.